File:Nijisanji Lineup February 2021.png

Summary
Nijisanji's entire roster of Japanese Virtual Livers as of February 2021.

From top to bottom, left to right:
 * Row 1 (top): Todo Kohaku, Suo Sango, Asahina Akane, Nishizono Chigusa, Kitakoji Hisui, Kaida Haru, Nagao Kei, Genzuki Tojiro, Furen E Lustario, Melissa Kinrenka, Ibrahim, Mashiro, Naraka, Kurusu Natsume, Hayama Marin, Nui Sociere, Sorahoshi Kirame
 * Row 2: Fuwa Minato, Shirayuki Tomoe, Gwelu Os Gar, Mayuzumi Kai, Ars Almal, Aiba Uiha, Yashiro Kizuku, Emma★August, Luis Cammy, Matsukai Mao, Fumi, Hoshikawa Sara, Yamagami Karuta, Yukishiro Mahiro, Suzuhara Lulu
 * Row 3: Ange Katrina, Inui Toko, Lize Helesta, Ex Albio, Saegusa Akina, Aizono Manami, Hanabatake Chaika, Warabeda Meiji, Otogibara Era, Yorumi Rena, Kagami Hayato, Hakase Fuyuki, Kanda Shoichi, Shellin Burgundy, Sukoya Kana, Hayase Sou
 * Row 4: Maimoto Keisuke, Gilzaren III, Levi Elipha, Ratna Petit, Amamiya Kokoro, Eli Conifer, Debidebi Debiru, Takamiya Rion, Joe Rikiichi, Rindou Mikoto, Gundo Mirei, Yuzuki Roa, Amemori Sayo, Yumeoi Kakeru, Kuroi Shiba
 * Row 5: Kataribe Tsumugu, Seto Miyako, Onomachi Haruka, Sakura Ritsuki, Belmond Banderas, Ryushen, Sister Claire, Dola, Kenmochi Toya, Fushimi Gaku, Asuka Hina, Machita Chima, Suzuki Masaru
 * Row 6: Naruse Naru, Harusaki Air, Todoroki Kyoko, Azuchi Momo, Suzuya Aki, Shibuya Hajime, Suzuka Utako, Ushimi Ichigo, Fumino Tamaki, Moira, Sasaki Saku, Shiina Yuika, Elu, Uzuki Kou, Yaguruma Rine
 * Row 7 (bottom): Kuzuha, Kanae, Akabane Youko, Morinaka Kazaki, Mononobe Alice, Shizuka Rin, Tsukino Mito, Higuchi Kaede, Yuuki Chihiro, Honma Himawari, Ienaga Mugi, Yuhi Riri, Makaino Ririmu

Source: https://www.ichikara.co.jp/service-nijisanji/